Journal: Development (Cambridge, England)

Article Title: Evolutionarily conserved requirement of Cdx for post-occipital tissue emergence.

doi: 10.1242/dev.079848

Figure Lengend Snippet: Fig. 5. Addition of Fgf8 to the whole embryo culture medium of Cdx2 null embryos rescues their axial elongation. (A,B)E8.0 (early somite) embryos at the start of the culture (A, wild type; B, Cdx2 null). (C-F)Embryos after their culture for the same period without (C,D) or with (E,F) Fgf8 added to the culture medium. C and E are controls; D and F are Cdx2 null mutants. (G-J)Mox1 expression in another set of Cdx2 null mutants (H,J) and controls (G,I) that have been cultured for the same period with (I,J) and without (G,H) Fgf8. (K)Comparison of posterior elongation of Cdx2 null and control embryos cultured without and with Fgf8. y axis, total number of somites generated in culture; bars on the graph represent median values; n, number of embryos; several experimental data are superimposed as one symbol in the graph as they had the same value. (L)Statistical analysis of the axial growth rescue of Cdx mutants by Fgf8 using the Mann-Whitney U test. al, allantois; flb, forelimb bud. Anterior is to the right in A,B and up in C-J. Scale bars: 0.5 mm. See also supplementary material Fig. S3.

Article Snippet: Recombinant Fgf8 (isoform b) was purchased from R&D Systems (423- F8).

Techniques: Embryo Culture, Expressing, Cell Culture, Comparison, Control, Generated, MANN-WHITNEY

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Mannan-Binding Lectin via Interaction With Cell Surface Calreticulin Promotes Senescence of Activated Hepatic Stellate Cells to Limit Liver Fibrosis Progression

doi: 10.1016/j.jcmgh.2022.03.011

Figure Lengend Snippet: MBL–CRT interaction up-regulates the association of CRT and LRP1 and downstream senescent-related signaling pathways in HSC. LX-2 cells were treated with CRT antibody or IgG 1 hour before the incubation of MBL and H 2 O 2 . ( A ) Representative photomicrographs of the SA–β-gal staining of LX-2 cells. ( B ) The distribution of cell cycle on the treated LX-2 cells with flow cytometry analysis. ( C ) The mRNA levels of senescence-related genes were assessed by quantitative reverse-transcription (qRT)-PCR analysis. ( D ) The association of CRT and LRP1 was determined by immunoblotting of immunoprecipitates in membrane fractions of LX-2 cells. ( E ) The association of LRP1, Rab8, and p110 was determined by immunoblotting of immunoprecipitates in cytoplasm fractions of LX-2 cells. ( F ) The p53, p21, mTOR, p-mTOR, AKT, p-AKT, and p110 protein levels were assessed by Western blot analysis. ( G ) The mRNA levels of fibrosis-associated genes were assessed by qRT-PCR analysis. Scale bars : 100 μm. Data are presented as means ± SEM. ∗∗ P < .01, Student t test or 1-way analysis of variance followed by Tukey post hoc tests for multiple group comparisons. The data shown represent 3 independent experiments. ATPase, adenosine triphosphatase; CCL, chemokine (C-C motif) ligand; CXCL, chemokine (C-X-C motif) ligand; HPF, high-power field; IL, interleukin; MMP, matrix metalloproteinase.

Article Snippet: PI3K antibody (21739-1-AP), Rab8 antibody (55296-1-AP), p53 antibody (60283-2-Ig), p21 antibody (10355-1-AP), p16 antibody (10883-1-AP), Bax antibody (50599-2-Ig), and Bcl2 antibody (12789-1-AP) were supplied by Proteintech (Chicago, IL).

Techniques: Protein-Protein interactions, Incubation, Staining, Flow Cytometry, Reverse Transcription, Quantitative RT-PCR, Western Blot, Membrane